Linked projectsNAKO-814

SummaryThe aim of this project is to conduct a deep proteomic profiling among participants of the NAKO study that were also part of the NAKO-IMMEDIATE dataset. Within the EU-funded IMMEDIATE project (https://www.immediate-project.eu/) we are currently investigating shared and non-shared inflammation sig-natures (based on OLINK® 96 inflammatory panel measured in baseline plasma) in the transition from health to disease in the domains of metabolic, cardiovascular, kidney and brain function in a subset of the NAKO Berlin-North study center participants (n=2,520), who have participated in the NAKO baseline examination (2014-2019) as well as in the first re-examination (2019-2024) (funding for these analyses was provided by Horizon Europe). Here, in a separate project, we propose to add a deep proteomics layer to these partici-pants by conducting an untargeted plasma proteome analysis using a mass spectrometry-based top 500 protein pipeline (Orbitrap Exploris 480 and Astral mass spectrometers) as well as nanoparticle-based plas-ma proteome profiling in both baseline and first re-examination samples. Results of this project will allow investigating shared and non-shared proteomics signatures predictive of the health to disease transition based on deep proteomics profiling at two time points.

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InstitutionsMax Delbrück Centrum für Molekulare Medizine, Max Delbrück Centrum für Molekulare Medizin (MDC), Max Delbrück Center for Molecular Medicine, Max-Delbrück-Centrum für Molekulare Medizin (MDC)